Recombinant Mouse Anti-EBV gH/gL Antibody (CL40)
CAT#: PABC-419
This product is a recombinant Mouse antibody that can recognize EBV gH/gL. CL40 can block membrane fusion with both B cells and epithelial cells. CL40 binding to gHgL/gp42 complexes is not blocked by gp42 and does not interfere with gp42 binding to HLA class II, indicating that its ability to block membrane fusion with B cells represents a defect in gB activation. CL40 binds to an epitope in gH D-II that overlaps substantially with a region observed to interact with the gp42 HP.
Tested Data
SDS-PAGE
Figure 1 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in SDS-PAGE
SDS-PAGE analysis of PABC-419 in non-reduced (Lane 2) and reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in SEC-HPLC
SEC-HPLC analysis of PABC-419 shows that the purity is more than 95%
WB
Figure 3 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in WB
Western blot analysis of PABC-419 was performed with Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
The secondary antibody: HRP Goat Anti-Mouse IgG (H+L)
DB
Figure 4 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in Dot Blot
Dot Blot analysis of PABC-419 was performed with Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag.
The secondary antibody: HRP Goat Anti-Mouse IgG (H+L)
SDS-PAGE
Figure 5 Anti-EBV gH/gL Antibody (PABC-419) in SDS-PAGE
SDS-PAGE analysis of PABC-419 in β-mercaptoethanol-reduced (Lane 1, 1.5 μg) and non-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
WB
Figure 6 Anti-EBV gH/gL Antibody (PABC-419) in WB
WB analysis of PABC-419 was performed by coating with Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer Protein (His & Flag Tag).
ELISA
Figure 7 Anti-EBV gH/gL Antibody (PABC-419) in ELISA
ELISA analysis of PABC-419 was performed by coating with Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer Protein (His & Flag Tag).
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- EBV gH/gL
- Species Reactivity
- EBV
- Clone
- CL40
- Applications
- ELISA, Inhib, FC, Neut
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Inhibition, Flow Cytometry and Neutralization. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- EBV gH/gL; Envelope glycoprotein H; Envelope glycoprotein L
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User-Friendly Procedure
The product had an easy-to-use protocol that makes learning how to utilize it efficiently simple. For someone who is unfamiliar with this particular antibody, this is a huge help.
Sturdy and Robust
During extended storage, this antibody fragment showed to be robust and stable. I saw no deterioration even after using it for several months.
Enhanced Intricacy and Precision
The affinity-purified nature increased the trustworthiness of the results in a variety of applications by enabling strong and accurate binding.
Q&As
-
How often are new lots of this antibody validated?
A: Thorough validation testing is performed on every new lot to guarantee the antibody performance's consistency and dependability.
-
For optimal effects, how should I keep this antibody?
A: For storage, keep the antibody at temperatures ranging from -20°C to -80°C. Repeated freeze-thaw cycles should be avoided since they weaken antibodies and limit their effectiveness in testing.
-
What is the antibody solution's concentration?
A: The antibody concentration is normally 1 mg/mL; however, it is always better to consult the product datasheet for specific information about the lot you are using.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-419, RRID: AB_3111590)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone CL40"
- CAT
- Product Name
See other products for "EBV gH/gL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-311 | Recombinant Human Anti-EBV gH/gL Antibody (AMMO1) | ELISA, BLI, Neut | Human IgG |
| PABC-523 | Mouse Anti-EBV gH/gL Recombinant Antibody (clone CL59) | ELISA, FC, Neut, Inhib | Mouse IgG1 |
| PABC-524 | Recombinant Mouse Anti-EBV gH/gL Antibody (E1D1) | ELISA, FC, Neut, Inhib | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-311 | Recombinant Human Anti-EBV gH/gL Antibody Fab Fragment (AMMO1) | ELISA, Neut | Human Fab |
| PFBC-419 | Recombinant Mouse Anti-EBV gH/gL Antibody Fab Fragment (CL40) | ELISA, FC, Neut | Mouse Fab |
| PFBC-523 | Recombinant Mouse Anti-EBV gH/gL Antibody Fab Fragment (CL59) | ELISA, FC, Neut | Mouse Fab |
| PFBC-524 | Recombinant Mouse Anti-EBV gH/gL Antibody Fab Fragment (E1D1) | ELISA, FC, Neut | Mouse Fab |
| PABC-523-F(E) | Mouse Anti-EBV gH/gL Recombinant Antibody (clone CL59); Fab Fragment | ELISA, FC, Neut, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-311 | Recombinant Human Anti-EBV gH/gL Antibody scFv Fragment (AMMO1) | ELISA, Neut | Human scFv |
| PSBC-419 | Recombinant Mouse Anti-EBV gH/gL Antibody scFv Fragment (CL40) | ELISA, FC, Neut | Mouse scFv |
| PSBC-523 | Recombinant Mouse Anti-EBV gH/gL Antibody scFv Fragment (CL59) | ELISA, FC, Neut | Mouse scFv |
| PSBC-524 | Recombinant Mouse Anti-EBV gH/gL Antibody scFv Fragment (E1D1) | ELISA, FC, Neut | Mouse scFv |
| PABC-523-S(P) | Mouse Anti-EBV gH/gL Recombinant Antibody (clone CL59); scFv Fragment | ELISA, FC, Neut, Inhib | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-XY12 | Human Anti-EBV gH/gL Vaccinal Antibody (VS-0625-XY12) | ELISA, Neut | Human IgG |
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